Engineering an Acinetobacter regulon for biosensing and high-throughput enzyme screening in E. coli via flow cytometry

We created a single cell sorting system to screen for enzyme activity in Escherichia coli producing 3,4 dihydroxy benzoate (34DHB). To do so, we engineered a transcription factor regulon controlling the expression of green fluorescent protein (GFP) for induction by 34DHB. An autoregulated transcript...

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Detalles Bibliográficos
Autores principales: Jha, Ramesh K., Kern, Theresa L., Fox, David T., M. Strauss, Charlie E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Synthetic Biology and Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081070/
https://www.ncbi.nlm.nih.gov/pubmed/24861620
http://dx.doi.org/10.1093/nar/gku444

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081070/
https://www.ncbi.nlm.nih.gov/pubmed/24861620
http://dx.doi.org/10.1093/nar/gku444

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