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Engineering an Acinetobacter regulon for biosensing and high-throughput enzyme screening in E. coli via flow cytometry
We created a single cell sorting system to screen for enzyme activity in Escherichia coli producing 3,4 dihydroxy benzoate (34DHB). To do so, we engineered a transcription factor regulon controlling the expression of green fluorescent protein (GFP) for induction by 34DHB. An autoregulated transcript...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081070/ https://www.ncbi.nlm.nih.gov/pubmed/24861620 http://dx.doi.org/10.1093/nar/gku444 |