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Correlated cryogenic photoactivated localization microscopy and electron cryotomography
Electron cryotomography (ECT) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated "cryo-PALM"-ECT method for localizing objects within cryotomograms to beyon...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081473/ https://www.ncbi.nlm.nih.gov/pubmed/24813625 http://dx.doi.org/10.1038/nmeth.2961 |