Correlated cryogenic photoactivated localization microscopy and electron cryotomography

Electron cryotomography (ECT) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated "cryo-PALM"-ECT method for localizing objects within cryotomograms to beyon...

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Detalles Bibliográficos
Autores principales: Chang, Yi-Wei, Chen, Songye, Tocheva, Elitza I., Treuner-Lange, Anke, Löbach, Stephanie, Søgaard-Andersen, Lotte, Jensen, Grant J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081473/
https://www.ncbi.nlm.nih.gov/pubmed/24813625
http://dx.doi.org/10.1038/nmeth.2961
Descripción
Sumario:Electron cryotomography (ECT) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated "cryo-PALM"-ECT method for localizing objects within cryotomograms to beyond the diffraction limit of the light microscope, and use it to identify multiple and new conformations of the dynamic type VI secretion system in the crowded interior of Myxococcus xanthus.

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