Correlated cryogenic photoactivated localization microscopy and electron cryotomography

Electron cryotomography (ECT) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated "cryo-PALM"-ECT method for localizing objects within cryotomograms to beyon...

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Detalles Bibliográficos
Autores principales: Chang, Yi-Wei, Chen, Songye, Tocheva, Elitza I., Treuner-Lange, Anke, Löbach, Stephanie, Søgaard-Andersen, Lotte, Jensen, Grant J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081473/
https://www.ncbi.nlm.nih.gov/pubmed/24813625
http://dx.doi.org/10.1038/nmeth.2961
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author Chang, Yi-Wei
Chen, Songye
Tocheva, Elitza I.
Treuner-Lange, Anke
Löbach, Stephanie
Søgaard-Andersen, Lotte
Jensen, Grant J.
author_facet Chang, Yi-Wei
Chen, Songye
Tocheva, Elitza I.
Treuner-Lange, Anke
Löbach, Stephanie
Søgaard-Andersen, Lotte
Jensen, Grant J.
author_sort Chang, Yi-Wei
collection PubMed
description Electron cryotomography (ECT) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated "cryo-PALM"-ECT method for localizing objects within cryotomograms to beyond the diffraction limit of the light microscope, and use it to identify multiple and new conformations of the dynamic type VI secretion system in the crowded interior of Myxococcus xanthus.
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spelling pubmed-40814732015-01-01 Correlated cryogenic photoactivated localization microscopy and electron cryotomography Chang, Yi-Wei Chen, Songye Tocheva, Elitza I. Treuner-Lange, Anke Löbach, Stephanie Søgaard-Andersen, Lotte Jensen, Grant J. Nat Methods Article Electron cryotomography (ECT) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated "cryo-PALM"-ECT method for localizing objects within cryotomograms to beyond the diffraction limit of the light microscope, and use it to identify multiple and new conformations of the dynamic type VI secretion system in the crowded interior of Myxococcus xanthus. 2014-05-11 2014-07 /pmc/articles/PMC4081473/ /pubmed/24813625 http://dx.doi.org/10.1038/nmeth.2961 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Chang, Yi-Wei
Chen, Songye
Tocheva, Elitza I.
Treuner-Lange, Anke
Löbach, Stephanie
Søgaard-Andersen, Lotte
Jensen, Grant J.
Correlated cryogenic photoactivated localization microscopy and electron cryotomography
title Correlated cryogenic photoactivated localization microscopy and electron cryotomography
title_full Correlated cryogenic photoactivated localization microscopy and electron cryotomography
title_fullStr Correlated cryogenic photoactivated localization microscopy and electron cryotomography
title_full_unstemmed Correlated cryogenic photoactivated localization microscopy and electron cryotomography
title_short Correlated cryogenic photoactivated localization microscopy and electron cryotomography
title_sort correlated cryogenic photoactivated localization microscopy and electron cryotomography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081473/
https://www.ncbi.nlm.nih.gov/pubmed/24813625
http://dx.doi.org/10.1038/nmeth.2961
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