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An electroporation protocol for efficient DNA transfection in PC12 cells

A wide variety of mammalian cell types is used in gene transfection studies. Establishing transfection methods that enable highly efficient DNA uptake has become increasingly important. PC12 is an established rat pheochromocytoma cell line, which responds to exposure to NGF with cessation of growth,...

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Detalles Bibliográficos
Autores principales: Covello, Giuseppina, Siva, Kavitha, Adami, Valentina, Denti, Michela A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4082779/
https://www.ncbi.nlm.nih.gov/pubmed/23846478
http://dx.doi.org/10.1007/s10616-013-9608-9