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Using a medium-throughput comet assay to evaluate the global DNA methylation status of single cells

The comet assay is a simple and cost effective technique, commonly used to analyze and quantify DNA damage in individual cells. The versatility of the comet assay allows introduction of various modifications to the basic technique. The difference in the methylation sensitivity of the isoschizomeric...

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Detalles Bibliográficos
Autores principales: Lewies, Angélique, Van Dyk, Etresia, Wentzel, Johannes F., Pretorius, Pieter J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083187/
https://www.ncbi.nlm.nih.gov/pubmed/25071840
http://dx.doi.org/10.3389/fgene.2014.00215