Structural insight into SUMO chain recognition and manipulation by the ubiquitin ligase RNF4

The small ubiquitin-like modifier (SUMO) can form polymeric chains that are important signals in cellular processes such as meiosis, genome maintenance and stress response. The SUMO-targeted ubiquitin ligase RNF4 engages with SUMO chains on linked substrates and catalyses their ubiquitination, which...

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Autores principales: Xu, Yingqi, Plechanovová, Anna, Simpson, Peter, Marchant, Jan, Leidecker, Orsolya, Kraatz, Sebastian, Hay, Ronald T., Matthews, Steve J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083429/
https://www.ncbi.nlm.nih.gov/pubmed/24969970
http://dx.doi.org/10.1038/ncomms5217
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author Xu, Yingqi
Plechanovová, Anna
Simpson, Peter
Marchant, Jan
Leidecker, Orsolya
Kraatz, Sebastian
Hay, Ronald T.
Matthews, Steve J.
author_facet Xu, Yingqi
Plechanovová, Anna
Simpson, Peter
Marchant, Jan
Leidecker, Orsolya
Kraatz, Sebastian
Hay, Ronald T.
Matthews, Steve J.
author_sort Xu, Yingqi
collection PubMed
description The small ubiquitin-like modifier (SUMO) can form polymeric chains that are important signals in cellular processes such as meiosis, genome maintenance and stress response. The SUMO-targeted ubiquitin ligase RNF4 engages with SUMO chains on linked substrates and catalyses their ubiquitination, which targets substrates for proteasomal degradation. Here we use a segmental labelling approach combined with solution nuclear magnetic resonance (NMR) spectroscopy and biochemical characterization to reveal how RNF4 manipulates the conformation of the SUMO chain, thereby facilitating optimal delivery of the distal SUMO domain for ubiquitin transfer.
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spelling pubmed-40834292014-07-09 Structural insight into SUMO chain recognition and manipulation by the ubiquitin ligase RNF4 Xu, Yingqi Plechanovová, Anna Simpson, Peter Marchant, Jan Leidecker, Orsolya Kraatz, Sebastian Hay, Ronald T. Matthews, Steve J. Nat Commun Article The small ubiquitin-like modifier (SUMO) can form polymeric chains that are important signals in cellular processes such as meiosis, genome maintenance and stress response. The SUMO-targeted ubiquitin ligase RNF4 engages with SUMO chains on linked substrates and catalyses their ubiquitination, which targets substrates for proteasomal degradation. Here we use a segmental labelling approach combined with solution nuclear magnetic resonance (NMR) spectroscopy and biochemical characterization to reveal how RNF4 manipulates the conformation of the SUMO chain, thereby facilitating optimal delivery of the distal SUMO domain for ubiquitin transfer. Nature Pub. Group 2014-06-27 /pmc/articles/PMC4083429/ /pubmed/24969970 http://dx.doi.org/10.1038/ncomms5217 Text en Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by-nc-by/3.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Xu, Yingqi
Plechanovová, Anna
Simpson, Peter
Marchant, Jan
Leidecker, Orsolya
Kraatz, Sebastian
Hay, Ronald T.
Matthews, Steve J.
Structural insight into SUMO chain recognition and manipulation by the ubiquitin ligase RNF4
title Structural insight into SUMO chain recognition and manipulation by the ubiquitin ligase RNF4
title_full Structural insight into SUMO chain recognition and manipulation by the ubiquitin ligase RNF4
title_fullStr Structural insight into SUMO chain recognition and manipulation by the ubiquitin ligase RNF4
title_full_unstemmed Structural insight into SUMO chain recognition and manipulation by the ubiquitin ligase RNF4
title_short Structural insight into SUMO chain recognition and manipulation by the ubiquitin ligase RNF4
title_sort structural insight into sumo chain recognition and manipulation by the ubiquitin ligase rnf4
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083429/
https://www.ncbi.nlm.nih.gov/pubmed/24969970
http://dx.doi.org/10.1038/ncomms5217
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