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Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR–Cas platform

The bacterial CRISPR/Cas system has proven to be an efficient gene-targeting tool in various organisms. Here we employ CRISPR/Cas for accurate and efficient genome editing in rats. The synthetic chimeric guide RNAs (gRNAs) discriminate a single-nucleotide polymorphism (SNP) difference in rat embryon...

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Detalles Bibliográficos
Autores principales: Yoshimi, K., Kaneko, T., Voigt, B., Mashimo, T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083438/
https://www.ncbi.nlm.nih.gov/pubmed/24967838
http://dx.doi.org/10.1038/ncomms5240