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Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency

In animal experiments, hippocampal neurogenesis and the activity of thiamine-dependent transketolase decrease markedly under conditions of thiamine deficiency. To further investigate the effect of thiamine deficiency on the proliferation of hippocampal progenitor cells (HPCs) and the potential mecha...

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Detalles Bibliográficos
Autores principales: Zhao, Yanling, Wu, Yiying, Hu, Haolu, Cai, Jinghui, Ning, Min, Ni, Xiushi, Zhong, Chunjiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083768/
https://www.ncbi.nlm.nih.gov/pubmed/25028661
http://dx.doi.org/10.1155/2014/572915
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author Zhao, Yanling
Wu, Yiying
Hu, Haolu
Cai, Jinghui
Ning, Min
Ni, Xiushi
Zhong, Chunjiu
author_facet Zhao, Yanling
Wu, Yiying
Hu, Haolu
Cai, Jinghui
Ning, Min
Ni, Xiushi
Zhong, Chunjiu
author_sort Zhao, Yanling
collection PubMed
description In animal experiments, hippocampal neurogenesis and the activity of thiamine-dependent transketolase decrease markedly under conditions of thiamine deficiency. To further investigate the effect of thiamine deficiency on the proliferation of hippocampal progenitor cells (HPCs) and the potential mechanisms involved in this effect, we cultured HPCs in vitro in the absence of thiamine and found that proliferation and transketolase activity were both significantly repressed. Furthermore, specific inhibition of transketolase activity by oxythiamine strongly inhibited HPC proliferation in a dose-dependent manner. However, thiamine deficiency itself inhibited the proliferation to a greater degree than did oxythiamine. Taken together, our results suggest that modulation of transketolase activity might be one of the mechanisms by which thiamine regulates the proliferation of hippocampal progenitor cells.
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spelling pubmed-40837682014-07-15 Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency Zhao, Yanling Wu, Yiying Hu, Haolu Cai, Jinghui Ning, Min Ni, Xiushi Zhong, Chunjiu Biomed Res Int Research Article In animal experiments, hippocampal neurogenesis and the activity of thiamine-dependent transketolase decrease markedly under conditions of thiamine deficiency. To further investigate the effect of thiamine deficiency on the proliferation of hippocampal progenitor cells (HPCs) and the potential mechanisms involved in this effect, we cultured HPCs in vitro in the absence of thiamine and found that proliferation and transketolase activity were both significantly repressed. Furthermore, specific inhibition of transketolase activity by oxythiamine strongly inhibited HPC proliferation in a dose-dependent manner. However, thiamine deficiency itself inhibited the proliferation to a greater degree than did oxythiamine. Taken together, our results suggest that modulation of transketolase activity might be one of the mechanisms by which thiamine regulates the proliferation of hippocampal progenitor cells. Hindawi Publishing Corporation 2014 2014-06-16 /pmc/articles/PMC4083768/ /pubmed/25028661 http://dx.doi.org/10.1155/2014/572915 Text en Copyright © 2014 Yanling Zhao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhao, Yanling
Wu, Yiying
Hu, Haolu
Cai, Jinghui
Ning, Min
Ni, Xiushi
Zhong, Chunjiu
Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency
title Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency
title_full Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency
title_fullStr Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency
title_full_unstemmed Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency
title_short Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency
title_sort downregulation of transketolase activity is related to inhibition of hippocampal progenitor cell proliferation induced by thiamine deficiency
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083768/
https://www.ncbi.nlm.nih.gov/pubmed/25028661
http://dx.doi.org/10.1155/2014/572915
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