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Spot quantification in two dimensional gel electrophoresis image analysis: comparison of different approaches and presentation of a novel compound fitting algorithm

BACKGROUND: Various computer-based methods exist for the detection and quantification of protein spots in two dimensional gel electrophoresis images. Area-based methods are commonly used for spot quantification: an area is assigned to each spot and the sum of the pixel intensities in that area, the...

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Detalles Bibliográficos
Autores principales: Brauner, Jan M, Groemer, Teja W, Stroebel, Armin, Grosse-Holz, Simon, Oberstein, Timo, Wiltfang, Jens, Kornhuber, Johannes, Maler, Juan Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085234/
https://www.ncbi.nlm.nih.gov/pubmed/24915860
http://dx.doi.org/10.1186/1471-2105-15-181