His-tag protein monitoring by a fast mix-and-measure immunoassay

Here, we present a fast mix-and-measure immunoassay for the specific semiquantitative detection of His-tagged proteins, for example in E. coli cell lysate. The assay is based on Förster resonance energy transfer (FRET) between a lanthanide dye-labeled low-affinity His-peptide and an acceptor-labeled...

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Detalles Bibliográficos
Autores principales: Kreisig, Thomas, Prasse, Agneta A., Zscharnack, Kristin, Volke, Daniela, Zuchner, Thole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085604/
https://www.ncbi.nlm.nih.gov/pubmed/25000910
http://dx.doi.org/10.1038/srep05613

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085604/
https://www.ncbi.nlm.nih.gov/pubmed/25000910
http://dx.doi.org/10.1038/srep05613

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