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Four Methods of Preparing mRNA 5′ End Libraries Using the Illumina Sequencing Platform
BACKGROUND: The 5′ untranslated regions of mRNA play an important role in their translation. RESULTS: Here, we describe the development of four methods of profiling mRNA 5′ ends using the Illumina sequencing platform; the first method utilizes SMART (Switching Mechanism At 5′ end of RNA Transcript)...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4086933/ https://www.ncbi.nlm.nih.gov/pubmed/25003736 http://dx.doi.org/10.1371/journal.pone.0101812 |