A rapid, site-selective and efficient route to the dual modification of DARPins

Designed ankyrin repeat proteins (DARPins) are valuable tools in both biochemistry and medicine. Herein we describe a rapid, simple method for the dual modification of DARPins by introduction of cysteine mutations at specific positions that results in a vast difference in their thiol nucleophilicity...

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Detalles Bibliográficos
Autores principales: Moody, Paul, Chudasama, Vijay, Nathani, Ramiz I., Maruani, Antoine, Martin, Stephen, Smith, Mark E. B., Caddick, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2014
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4091302/
https://www.ncbi.nlm.nih.gov/pubmed/24687090
http://dx.doi.org/10.1039/c4cc00053f

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4091302/
https://www.ncbi.nlm.nih.gov/pubmed/24687090
http://dx.doi.org/10.1039/c4cc00053f

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