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Production of Mutated Porcine Embryos Using Zinc Finger Nucleases and a Reporter-based Cell Enrichment System

To facilitate the construction of genetically-modified pigs, we produced cloned embryos derived from porcine fibroblasts transfected with a pair of engineered zinc finger nuclease (ZFN) plasmids to create targeted mutations and enriched using a reporter plasmid system. The reporter expresses RFP and...

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Detalles Bibliográficos
Autores principales: Koo, Ok Jae, Park, Sol Ji, Lee, Choongil, Kang, Jung Taek, Kim, Sujin, Moon, Joon Ho, Choi, Ji Yei, Kim, Hyojin, Jang, Goo, Kim, Jin-Soo, Kim, Seokjoong, Lee, Byeong-Chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4093273/
https://www.ncbi.nlm.nih.gov/pubmed/25049958
http://dx.doi.org/10.5713/ajas.2013.13481