Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc

[Image: see text] We report the synthesis and photophysical properties of ZBR4 and ZR1, two resorufin-based ditopic probes for mobile zinc. Upon binding Zn(2+), the sensors display 14- and 41-fold enhancements of their red fluorescence emission, respectively. In contrast to ZR1 and other members of...

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Autores principales: Loas, Andrei, Radford, Robert J., Lippard, Stephen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4095928/
https://www.ncbi.nlm.nih.gov/pubmed/24915285
http://dx.doi.org/10.1021/ic500732z
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author Loas, Andrei
Radford, Robert J.
Lippard, Stephen J.
author_facet Loas, Andrei
Radford, Robert J.
Lippard, Stephen J.
author_sort Loas, Andrei
collection PubMed
description [Image: see text] We report the synthesis and photophysical properties of ZBR4 and ZR1, two resorufin-based ditopic probes for mobile zinc. Upon binding Zn(2+), the sensors display 14- and 41-fold enhancements of their red fluorescence emission, respectively. In contrast to ZR1 and other members of the ZBR family, which accumulate in the endoplasmic reticulum, ZBR4 spontaneously localizes to the mitochondria of HeLa cells. The modular approach in designing the constructs facilitates a homologation strategy aimed at tuning the zinc-binding and intracellular targeting properties of future probes.
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spelling pubmed-40959282014-07-24 Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc Loas, Andrei Radford, Robert J. Lippard, Stephen J. Inorg Chem [Image: see text] We report the synthesis and photophysical properties of ZBR4 and ZR1, two resorufin-based ditopic probes for mobile zinc. Upon binding Zn(2+), the sensors display 14- and 41-fold enhancements of their red fluorescence emission, respectively. In contrast to ZR1 and other members of the ZBR family, which accumulate in the endoplasmic reticulum, ZBR4 spontaneously localizes to the mitochondria of HeLa cells. The modular approach in designing the constructs facilitates a homologation strategy aimed at tuning the zinc-binding and intracellular targeting properties of future probes. American Chemical Society 2014-06-10 2014-07-07 /pmc/articles/PMC4095928/ /pubmed/24915285 http://dx.doi.org/10.1021/ic500732z Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Loas, Andrei
Radford, Robert J.
Lippard, Stephen J.
Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc
title Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc
title_full Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc
title_fullStr Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc
title_full_unstemmed Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc
title_short Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc
title_sort addition of a second binding site increases the dynamic range but alters the cellular localization of a red fluorescent probe for mobile zinc
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4095928/
https://www.ncbi.nlm.nih.gov/pubmed/24915285
http://dx.doi.org/10.1021/ic500732z
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AT radfordrobertj additionofasecondbindingsiteincreasesthedynamicrangebutaltersthecellularlocalizationofaredfluorescentprobeformobilezinc
AT lippardstephenj additionofasecondbindingsiteincreasesthedynamicrangebutaltersthecellularlocalizationofaredfluorescentprobeformobilezinc

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