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Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates....

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Detalles Bibliográficos
Autores principales: Bossé, Janine T., Li, Yanwen, Angen, Øystein, Weinert, Lucy A., Chaudhuri, Roy R., Holden, Matthew T., Williamson, Susanna M., Maskell, Duncan J., Tucker, Alexander W., Wren, Brendan W., Rycroft, Andrew N., Langford, Paul R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4097740/
https://www.ncbi.nlm.nih.gov/pubmed/24759717
http://dx.doi.org/10.1128/JCM.00685-14