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Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12
An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates....
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society for Microbiology
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4097740/ https://www.ncbi.nlm.nih.gov/pubmed/24759717 http://dx.doi.org/10.1128/JCM.00685-14 |
| _version_ | 1782326240206651392 |
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| author | Bossé, Janine T. Li, Yanwen Angen, Øystein Weinert, Lucy A. Chaudhuri, Roy R. Holden, Matthew T. Williamson, Susanna M. Maskell, Duncan J. Tucker, Alexander W. Wren, Brendan W. Rycroft, Andrew N. Langford, Paul R. |
| author_facet | Bossé, Janine T. Li, Yanwen Angen, Øystein Weinert, Lucy A. Chaudhuri, Roy R. Holden, Matthew T. Williamson, Susanna M. Maskell, Duncan J. Tucker, Alexander W. Wren, Brendan W. Rycroft, Andrew N. Langford, Paul R. |
| author_sort | Bossé, Janine T. |
| collection | PubMed |
| description | An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates. |
| format | Online Article Text |
| id | pubmed-4097740 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2014 |
| publisher | American Society for Microbiology |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-40977402014-07-25 Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12 Bossé, Janine T. Li, Yanwen Angen, Øystein Weinert, Lucy A. Chaudhuri, Roy R. Holden, Matthew T. Williamson, Susanna M. Maskell, Duncan J. Tucker, Alexander W. Wren, Brendan W. Rycroft, Andrew N. Langford, Paul R. J Clin Microbiol Clinical Veterinary Microbiology An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates. American Society for Microbiology 2014-07 /pmc/articles/PMC4097740/ /pubmed/24759717 http://dx.doi.org/10.1128/JCM.00685-14 Text en Copyright © 2014 Bossé et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) . |
| spellingShingle | Clinical Veterinary Microbiology Bossé, Janine T. Li, Yanwen Angen, Øystein Weinert, Lucy A. Chaudhuri, Roy R. Holden, Matthew T. Williamson, Susanna M. Maskell, Duncan J. Tucker, Alexander W. Wren, Brendan W. Rycroft, Andrew N. Langford, Paul R. Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12 |
| title | Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12 |
| title_full | Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12 |
| title_fullStr | Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12 |
| title_full_unstemmed | Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12 |
| title_short | Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12 |
| title_sort | multiplex pcr assay for unequivocal differentiation of actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12 |
| topic | Clinical Veterinary Microbiology |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4097740/ https://www.ncbi.nlm.nih.gov/pubmed/24759717 http://dx.doi.org/10.1128/JCM.00685-14 |
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