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Visualization of Phosphatidic Acid Fluctuations in the Plasma Membrane of Living Cells

We developed genetically-encoded fluorescent sensors based on Förster Resonance Energy Transfer to monitor phosphatidic acid (PA) fluctuations in the plasma membrane using Spo20 as PA-binding motif. Basal PA levels and phospholipase D activity varied in different cell types. In addition, stimuli tha...

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Detalles Bibliográficos
Autores principales:	Ferraz-Nogueira, José P., Díez-Guerra, F. Javier, Llopis, Juan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099201/ https://www.ncbi.nlm.nih.gov/pubmed/25025521 http://dx.doi.org/10.1371/journal.pone.0102526

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