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Application of Gaussia luciferase in bicistronic and non-conventional secretion reporter constructs

BACKGROUND: Secreted luciferases are highly useful bioluminescent reporters for cell-based assays and drug discovery. A variety of secreted luciferases from marine organisms have been described that harbor an N-terminal signal peptide for release along the classical secretory pathway. Here, we have...

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Detalles Bibliográficos
Autores principales: Luft, Christin, Freeman, Jamie, Elliott, David, Al-Tamimi, Nadia, Kriston-Vizi, Janos, Heintze, Jacob, Lindenschmidt, Ida, Seed, Brian, Ketteler, Robin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4099409/
https://www.ncbi.nlm.nih.gov/pubmed/25007711
http://dx.doi.org/10.1186/1471-2091-15-14