Role of Candida albicans-Secreted Aspartyl Proteinases (Saps) in Severe Early Childhood Caries

Candida albicans is strongly associated with severe early childhood caries (S-ECC). However, the roles of secreted aspartyl proteinases (Saps), an important virulence factor of C. albicans, in the progress of S-ECC are not clear. In our study, the Saps activities were evaluated by the yeast nitrogen...

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Autores principales: Li, Wenqing, Yu, Dongsheng, Gao, Shuo, Lin, Jiacheng, Chen, Zhuoyu, Zhao, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100179/
https://www.ncbi.nlm.nih.gov/pubmed/24933640
http://dx.doi.org/10.3390/ijms150610766
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author Li, Wenqing
Yu, Dongsheng
Gao, Shuo
Lin, Jiacheng
Chen, Zhuoyu
Zhao, Wei
author_facet Li, Wenqing
Yu, Dongsheng
Gao, Shuo
Lin, Jiacheng
Chen, Zhuoyu
Zhao, Wei
author_sort Li, Wenqing
collection PubMed
description Candida albicans is strongly associated with severe early childhood caries (S-ECC). However, the roles of secreted aspartyl proteinases (Saps), an important virulence factor of C. albicans, in the progress of S-ECC are not clear. In our study, the Saps activities were evaluated by the yeast nitrogen base–bovine serum albumi (YNB–BSA) agar plate method and by the MTT method with bovine serum albumin (BSA) as the substrate. Genotypes of C. albicans and gene expression of Sap1–5 were evaluated. The relationships of Saps activities and genotypes with S-ECC were analyzed. The results showed that enzyme activities of Saps in the S-ECC group were significantly higher than those in the caries free (CF) group (p < 0.05). Genotypes A, B and C were detected in the S-ECC group, and genotypes A and C were detected in the CF group. In the genotype A group, Saps activity in the S-ECC group was significantly different from that in the CF group (p < 0.05). The gene expression level of Sap1 in the S-ECC group was significantly higher than that in the CF group (p = 0.001), while Sap4 expression was significantly lower than that in the CF group (p = 0.029). It can be concluded that Sap1–5 are the predominant proteinase genes expressed in C. albicans from dental biofilm and Sap1 may play an important role in the development of S-ECC.
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spelling pubmed-41001792014-07-16 Role of Candida albicans-Secreted Aspartyl Proteinases (Saps) in Severe Early Childhood Caries Li, Wenqing Yu, Dongsheng Gao, Shuo Lin, Jiacheng Chen, Zhuoyu Zhao, Wei Int J Mol Sci Article Candida albicans is strongly associated with severe early childhood caries (S-ECC). However, the roles of secreted aspartyl proteinases (Saps), an important virulence factor of C. albicans, in the progress of S-ECC are not clear. In our study, the Saps activities were evaluated by the yeast nitrogen base–bovine serum albumi (YNB–BSA) agar plate method and by the MTT method with bovine serum albumin (BSA) as the substrate. Genotypes of C. albicans and gene expression of Sap1–5 were evaluated. The relationships of Saps activities and genotypes with S-ECC were analyzed. The results showed that enzyme activities of Saps in the S-ECC group were significantly higher than those in the caries free (CF) group (p < 0.05). Genotypes A, B and C were detected in the S-ECC group, and genotypes A and C were detected in the CF group. In the genotype A group, Saps activity in the S-ECC group was significantly different from that in the CF group (p < 0.05). The gene expression level of Sap1 in the S-ECC group was significantly higher than that in the CF group (p = 0.001), while Sap4 expression was significantly lower than that in the CF group (p = 0.029). It can be concluded that Sap1–5 are the predominant proteinase genes expressed in C. albicans from dental biofilm and Sap1 may play an important role in the development of S-ECC. MDPI 2014-06-13 /pmc/articles/PMC4100179/ /pubmed/24933640 http://dx.doi.org/10.3390/ijms150610766 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Li, Wenqing
Yu, Dongsheng
Gao, Shuo
Lin, Jiacheng
Chen, Zhuoyu
Zhao, Wei
Role of Candida albicans-Secreted Aspartyl Proteinases (Saps) in Severe Early Childhood Caries
title Role of Candida albicans-Secreted Aspartyl Proteinases (Saps) in Severe Early Childhood Caries
title_full Role of Candida albicans-Secreted Aspartyl Proteinases (Saps) in Severe Early Childhood Caries
title_fullStr Role of Candida albicans-Secreted Aspartyl Proteinases (Saps) in Severe Early Childhood Caries
title_full_unstemmed Role of Candida albicans-Secreted Aspartyl Proteinases (Saps) in Severe Early Childhood Caries
title_short Role of Candida albicans-Secreted Aspartyl Proteinases (Saps) in Severe Early Childhood Caries
title_sort role of candida albicans-secreted aspartyl proteinases (saps) in severe early childhood caries
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100179/
https://www.ncbi.nlm.nih.gov/pubmed/24933640
http://dx.doi.org/10.3390/ijms150610766
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