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Improved Yield of High Molecular Weight DNA Coincides with Increased Microbial Diversity Access from Iron Oxide Cemented Sub-Surface Clay Environments

Despite over three decades of progress, extraction of high molecular weight (HMW) DNA from high clay soils or iron oxide cemented clay has remained challenging. HMW DNA is desirable for next generation sequencing as it yields the most comprehensive coverage. Several DNA extraction procedures were co...

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Detalles Bibliográficos
Autores principales: Hurt, Richard A., Robeson, Michael S., Shakya, Migun, Moberly, James G., Vishnivetskaya, Tatiana A., Gu, Baohua, Elias, Dwayne A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102596/
https://www.ncbi.nlm.nih.gov/pubmed/25033199
http://dx.doi.org/10.1371/journal.pone.0102826