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Improved Yield of High Molecular Weight DNA Coincides with Increased Microbial Diversity Access from Iron Oxide Cemented Sub-Surface Clay Environments
Despite over three decades of progress, extraction of high molecular weight (HMW) DNA from high clay soils or iron oxide cemented clay has remained challenging. HMW DNA is desirable for next generation sequencing as it yields the most comprehensive coverage. Several DNA extraction procedures were co...
Autores principales: | Hurt, Richard A., Robeson, Michael S., Shakya, Migun, Moberly, James G., Vishnivetskaya, Tatiana A., Gu, Baohua, Elias, Dwayne A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102596/ https://www.ncbi.nlm.nih.gov/pubmed/25033199 http://dx.doi.org/10.1371/journal.pone.0102826 |
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