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Long-range PCR in next-generation sequencing: comparison of six enzymes and evaluation on the MiSeq sequencer

Long-range PCR remains a flexible, fast, efficient and cost-effective choice for sequencing candidate genomic regions in a small number of samples, especially when combined with next-generation sequencing (NGS) platforms. Several long-range DNA polymerases are advertised as being able to amplify up...

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Detalles Bibliográficos
Autores principales: Jia, Haiying, Guo, Yunfei, Zhao, Weiwei, Wang, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102922/
https://www.ncbi.nlm.nih.gov/pubmed/25034901
http://dx.doi.org/10.1038/srep05737