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Long-range PCR in next-generation sequencing: comparison of six enzymes and evaluation on the MiSeq sequencer
Long-range PCR remains a flexible, fast, efficient and cost-effective choice for sequencing candidate genomic regions in a small number of samples, especially when combined with next-generation sequencing (NGS) platforms. Several long-range DNA polymerases are advertised as being able to amplify up...
Autores principales: | Jia, Haiying, Guo, Yunfei, Zhao, Weiwei, Wang, Kai |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102922/ https://www.ncbi.nlm.nih.gov/pubmed/25034901 http://dx.doi.org/10.1038/srep05737 |
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