Development and characterization of microsatellite markers for the endangered Amazonian tree Aniba rosaeodora (Lauraceae)(1)

• Premise of the study: Microsatellite loci were isolated and characterized for Brazilian rosewood (Aniba rosaeodora), an endangered neotropical hardwood tree, to investigate population and conservation genetics of this highly valuable nontimber forest resource. • Methods and Results: We used an enr...

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Detalles Bibliográficos
Autores principales: Angrizani, Rafael C., Contim, Luis A. S., Lemes, Maristerra R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103142/
https://www.ncbi.nlm.nih.gov/pubmed/25202581
http://dx.doi.org/10.3732/apps.1200516
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author Angrizani, Rafael C.
Contim, Luis A. S.
Lemes, Maristerra R.
author_facet Angrizani, Rafael C.
Contim, Luis A. S.
Lemes, Maristerra R.
author_sort Angrizani, Rafael C.
collection PubMed
description • Premise of the study: Microsatellite loci were isolated and characterized for Brazilian rosewood (Aniba rosaeodora), an endangered neotropical hardwood tree, to investigate population and conservation genetics of this highly valuable nontimber forest resource. • Methods and Results: We used an enriched genomic library method to isolate and characterize 11 nuclear microsatellite loci for A. rosaeodora, which exhibited an average of 9.6 and 8.7 alleles per locus in two populations from central Amazonia. Mean observed and expected heterozygosities over the 11 loci were 0.604 and 0.687, and 0.807 and 0.828, respectively, in the two populations. • Conclusions: The polymorphic microsatellite loci developed for A. rosaeodora showed highly informative content and can be used as a powerful tool in genetic diversity and population structure, gene flow, and mating system studies for conservation purposes.
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spelling pubmed-41031422014-09-08 Development and characterization of microsatellite markers for the endangered Amazonian tree Aniba rosaeodora (Lauraceae)(1) Angrizani, Rafael C. Contim, Luis A. S. Lemes, Maristerra R. Appl Plant Sci Primer Note • Premise of the study: Microsatellite loci were isolated and characterized for Brazilian rosewood (Aniba rosaeodora), an endangered neotropical hardwood tree, to investigate population and conservation genetics of this highly valuable nontimber forest resource. • Methods and Results: We used an enriched genomic library method to isolate and characterize 11 nuclear microsatellite loci for A. rosaeodora, which exhibited an average of 9.6 and 8.7 alleles per locus in two populations from central Amazonia. Mean observed and expected heterozygosities over the 11 loci were 0.604 and 0.687, and 0.807 and 0.828, respectively, in the two populations. • Conclusions: The polymorphic microsatellite loci developed for A. rosaeodora showed highly informative content and can be used as a powerful tool in genetic diversity and population structure, gene flow, and mating system studies for conservation purposes. Botanical Society of America 2013-09-02 /pmc/articles/PMC4103142/ /pubmed/25202581 http://dx.doi.org/10.3732/apps.1200516 Text en © 2013 Botanical Society of America
spellingShingle Primer Note
Angrizani, Rafael C.
Contim, Luis A. S.
Lemes, Maristerra R.
Development and characterization of microsatellite markers for the endangered Amazonian tree Aniba rosaeodora (Lauraceae)(1)
title Development and characterization of microsatellite markers for the endangered Amazonian tree Aniba rosaeodora (Lauraceae)(1)
title_full Development and characterization of microsatellite markers for the endangered Amazonian tree Aniba rosaeodora (Lauraceae)(1)
title_fullStr Development and characterization of microsatellite markers for the endangered Amazonian tree Aniba rosaeodora (Lauraceae)(1)
title_full_unstemmed Development and characterization of microsatellite markers for the endangered Amazonian tree Aniba rosaeodora (Lauraceae)(1)
title_short Development and characterization of microsatellite markers for the endangered Amazonian tree Aniba rosaeodora (Lauraceae)(1)
title_sort development and characterization of microsatellite markers for the endangered amazonian tree aniba rosaeodora (lauraceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103142/
https://www.ncbi.nlm.nih.gov/pubmed/25202581
http://dx.doi.org/10.3732/apps.1200516
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