An efficient technique for primer development and application that integrates fluorescent labeling and multiplex PCR(1)

• Premise of the study: Development of genetic markers can be costly and time-consuming, especially when multiple primer pairs are fluorescently labeled. This step was streamlined by combining two techniques in the same PCR reaction: (1) custom-labeling of primers by the investigator and (2) multipl...

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Detalles Bibliográficos
Autores principales: Culley, Theresa M., Stamper, Trevor I., Stokes, Richard L., Brzyski, Jessica R., Hardiman, Nicole A., Klooster, Matthew R., Merritt, Benjamin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2013
Materias:
Protocol Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103466/
https://www.ncbi.nlm.nih.gov/pubmed/25202486
http://dx.doi.org/10.3732/apps.1300027

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103466/
https://www.ncbi.nlm.nih.gov/pubmed/25202486
http://dx.doi.org/10.3732/apps.1300027

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