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An efficient technique for primer development and application that integrates fluorescent labeling and multiplex PCR(1)
• Premise of the study: Development of genetic markers can be costly and time-consuming, especially when multiple primer pairs are fluorescently labeled. This step was streamlined by combining two techniques in the same PCR reaction: (1) custom-labeling of primers by the investigator and (2) multipl...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Botanical Society of America
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103466/ https://www.ncbi.nlm.nih.gov/pubmed/25202486 http://dx.doi.org/10.3732/apps.1300027 |