Residues Controlling Facial Selectivity in an Alkene Reductase and Semirational Alterations to Create Stereocomplementary Variants

[Image: see text] A systematic saturation mutagenesis campaign was carried out on an alkene reductase from Pichia stipitis (OYE 2.6) to develop variants with reversed stereoselectivities. Wild-type OYE 2.6 reduces three representative Baylis–Hillman adducts to the corresponding S products with almos...

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Detalles Bibliográficos
Autores principales: Walton, Adam Z., Sullivan, Bradford, Patterson-Orazem, Athéna C., Stewart, Jon D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105185/
https://www.ncbi.nlm.nih.gov/pubmed/25068071
http://dx.doi.org/10.1021/cs500429k

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105185/
https://www.ncbi.nlm.nih.gov/pubmed/25068071
http://dx.doi.org/10.1021/cs500429k

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