Two Structurally Different Dienelactone Hydrolases (TfdEI and TfdEII) from Cupriavidus necator JMP134 Plasmid pJP4 Catalyse Cis- and Trans-Dienelactones with Similar Efficiency

In this study, dienelactone hydrolases (TfdEI and TfdEII) located on plasmid pJP4 of Cupriavidus necator JMP134 were cloned, purified, characterized and three dimensional structures were predicted. tfdEI and tfdEII genes were cloned into pET21b vector and expressed in E. coli BL21(DE3). The enzymes...

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Detalles Bibliográficos
Autores principales: Kumar, Ajit, Pillay, Balakrishna, Olaniran, Ademola O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4108320/
https://www.ncbi.nlm.nih.gov/pubmed/25054964
http://dx.doi.org/10.1371/journal.pone.0101801

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4108320/
https://www.ncbi.nlm.nih.gov/pubmed/25054964
http://dx.doi.org/10.1371/journal.pone.0101801

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