Antibody-based depletion of Foxp3+ T cells potentiates antitumor immune memory stimulated by mTOR inhibition

Inhibition of mTOR signaling enhances antitumor memory T lymphocytes while increasing the frequency of immunosuppressive regulatory T cells (Tregs). We report here a strategy to further improve immunologic memory by controlling CD4(+) T cells with CD4-depleting monoclonal antibody therapy thereby improving CD8(+) memory T cell quality and function. We report that removal of Tregs is the mechanism underlying immunological memory formation in response to this combination immunotherapy.