New methods as alternative or corrective measures for the pitfalls and artifacts of reverse transcription and polymerase chain reactions (RT-PCR) in cloning chimeric or antisense-accompanied RNA

We established new methods for cloning cDNA ends that start with reverse transcription (RT) and soon proceed with the synthesis of the second cDNA strand, avoiding manipulations of fragile RNA. Our 3′-end cloning method does not involve poly-dT primers and polymerase chain reactions (PCR), is low in...

Descripción completa

Detalles Bibliográficos
Autores principales: Yuan, Chengfu, Liu, Yongming, Yang, Min, Liao, D. Joshua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Technical Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4111735/
https://www.ncbi.nlm.nih.gov/pubmed/23618925
http://dx.doi.org/10.4161/rna.24570

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4111735/
https://www.ncbi.nlm.nih.gov/pubmed/23618925
http://dx.doi.org/10.4161/rna.24570

Ejemplares similares