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3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells

Synthetic 3′-biotin-tagged microRNAs (miRNAs) have often been used to select interacting messenger RNA (mRNA) and noncoding RNA (ncRNA) targets. Here, we examined the extent of association of 3′-end biotinylated miR-27 with Argonaute (Ago) proteins in transfected human cells using a coimmunoprecipit...

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Detalles Bibliográficos
Autores principales: Guo, Yang Eric, Steitz, Joan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4114695/
https://www.ncbi.nlm.nih.gov/pubmed/24821854
http://dx.doi.org/10.1261/rna.045054.114