3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells

Synthetic 3′-biotin-tagged microRNAs (miRNAs) have often been used to select interacting messenger RNA (mRNA) and noncoding RNA (ncRNA) targets. Here, we examined the extent of association of 3′-end biotinylated miR-27 with Argonaute (Ago) proteins in transfected human cells using a coimmunoprecipit...

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Detalles Bibliográficos
Autores principales: Guo, Yang Eric, Steitz, Joan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4114695/
https://www.ncbi.nlm.nih.gov/pubmed/24821854
http://dx.doi.org/10.1261/rna.045054.114
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author Guo, Yang Eric
Steitz, Joan A.
author_facet Guo, Yang Eric
Steitz, Joan A.
author_sort Guo, Yang Eric
collection PubMed
description Synthetic 3′-biotin-tagged microRNAs (miRNAs) have often been used to select interacting messenger RNA (mRNA) and noncoding RNA (ncRNA) targets. Here, we examined the extent of association of 3′-end biotinylated miR-27 with Argonaute (Ago) proteins in transfected human cells using a coimmunoprecipitation assay followed by Northern blot analysis. We report that biotinylated miR-27 does not efficiently associate with Ago compared to unmodified miR-27. These results suggest that 3′-end biotin-modified miRNAs are questionable monitors of miRNA function in cells.
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spelling pubmed-41146952015-07-01 3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells Guo, Yang Eric Steitz, Joan A. RNA Letter to the Editor Synthetic 3′-biotin-tagged microRNAs (miRNAs) have often been used to select interacting messenger RNA (mRNA) and noncoding RNA (ncRNA) targets. Here, we examined the extent of association of 3′-end biotinylated miR-27 with Argonaute (Ago) proteins in transfected human cells using a coimmunoprecipitation assay followed by Northern blot analysis. We report that biotinylated miR-27 does not efficiently associate with Ago compared to unmodified miR-27. These results suggest that 3′-end biotin-modified miRNAs are questionable monitors of miRNA function in cells. Cold Spring Harbor Laboratory Press 2014-07 /pmc/articles/PMC4114695/ /pubmed/24821854 http://dx.doi.org/10.1261/rna.045054.114 Text en © 2014 Guo and Steitz; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Letter to the Editor
Guo, Yang Eric
Steitz, Joan A.
3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells
title 3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells
title_full 3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells
title_fullStr 3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells
title_full_unstemmed 3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells
title_short 3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells
title_sort 3′-biotin-tagged microrna-27 does not associate with argonaute proteins in cells
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4114695/
https://www.ncbi.nlm.nih.gov/pubmed/24821854
http://dx.doi.org/10.1261/rna.045054.114
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