Cargando…
3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells
Synthetic 3′-biotin-tagged microRNAs (miRNAs) have often been used to select interacting messenger RNA (mRNA) and noncoding RNA (ncRNA) targets. Here, we examined the extent of association of 3′-end biotinylated miR-27 with Argonaute (Ago) proteins in transfected human cells using a coimmunoprecipit...
Autores principales: | Guo, Yang Eric, Steitz, Joan A. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4114695/ https://www.ncbi.nlm.nih.gov/pubmed/24821854 http://dx.doi.org/10.1261/rna.045054.114 |
Ejemplares similares
-
Homeostatic control of Argonaute stability by microRNA availability
por: Smibert, Peter, et al.
Publicado: (2013) -
Intertwined pathways for Argonaute-mediated microRNA biogenesis in Drosophila
por: Yang, Jr-Shiuan, et al.
Publicado: (2014) -
Primary microRNA transcript retention at sites of transcription leads to enhanced microRNA production
por: Pawlicki, Jan M., et al.
Publicado: (2008) -
microRNAs associated with the different human Argonaute proteins
por: Dueck, Anne, et al.
Publicado: (2012) -
Comment on “High doses of biotin can interfere with immunoassays that use biotin-strept(avidin) technologies: Implications for individuals with biotin-responsive inherited metabolic disorders”
por: Favresse, Julien, et al.
Publicado: (2019)