The “Gate Keeper” Role of Trp222 Determines the Enantiopreference of Diketoreductase toward 2-Chloro-1-Phenylethanone

Trp222 of diketoreductase (DKR), an enzyme responsible for reducing a variety of ketones to chiral alcohols, is located at the hydrophobic dimeric interface of the C-terminus. Single substitutions at DKR Trp222 with either canonical (Val, Leu, Met, Phe and Tyr) or unnatural amino acids (UAAs) (4-cya...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Hairong, Yang, Xin, Lu, Zhuo, Liu, Nan, Chen, Yijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4114983/
https://www.ncbi.nlm.nih.gov/pubmed/25072248
http://dx.doi.org/10.1371/journal.pone.0103792

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4114983/
https://www.ncbi.nlm.nih.gov/pubmed/25072248
http://dx.doi.org/10.1371/journal.pone.0103792

Ejemplares similares