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Single molecular investigation of DNA looping and aggregation by restriction endonuclease BspMI

DNA looping and aggregation induced by restriction endonuclease BspMI are studied by atomic force microscopy (AFM) and magnetic tweezers (MT). With Ca(2+) substituted for the normal enzyme cofactor Mg(2+) and enzyme concentration below the critical concentration of 6 units/mL, AFM images of DNA-BspM...

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Detalles Bibliográficos
Autores principales: Wang, Yanwei, Ran, Shiyong, Yang, Guangcan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4116625/
https://www.ncbi.nlm.nih.gov/pubmed/25077775
http://dx.doi.org/10.1038/srep05897