Autoradiography screening of potential positron emission tomography tracers for asymptomatic abdominal aortic aneurysms

OBJECTIVE: The aetiology and early pathophysiological mechanisms of aortic aneurysm formation are still unknown and challenging to study in vivo. Positron emission tomography (PET) is a potentially valuable instrument for non-invasive in vivo pathophysiological studies. No specific tracer to identif...

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Autores principales: Tegler, Gustaf, Estrada, Sergio, Hall, Håkan, Wanhainen, Anders, Björck, Martin, Sörensen, Jens, Antoni, Gunnar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Informa Healthcare 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4116762/
https://www.ncbi.nlm.nih.gov/pubmed/24555564
http://dx.doi.org/10.3109/03009734.2014.894157
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author Tegler, Gustaf
Estrada, Sergio
Hall, Håkan
Wanhainen, Anders
Björck, Martin
Sörensen, Jens
Antoni, Gunnar
author_facet Tegler, Gustaf
Estrada, Sergio
Hall, Håkan
Wanhainen, Anders
Björck, Martin
Sörensen, Jens
Antoni, Gunnar
author_sort Tegler, Gustaf
collection PubMed
description OBJECTIVE: The aetiology and early pathophysiological mechanisms of aortic aneurysm formation are still unknown and challenging to study in vivo. Positron emission tomography (PET) is a potentially valuable instrument for non-invasive in vivo pathophysiological studies. No specific tracer to identify the pathophysiological process of aneurysmal dilatation is yet available, however. The aim of this study was to explore if different PET tracers could be useful to image aneurysmal disease. METHODS AND RESULTS: Human aneurysmal aortic tissue, collected during elective resection of abdominal aortic aneurysm (AAA) of asymptomatic patients, was investigated in vitro by means of autoradiography with [(68)Ga]CRP-binder targeting C-reactive protein, [(11)C]DAA1106 targeting translocator protein (18 kDa), [(11)C]D-deprenyl with unknown target receptor, [(11)C]deuterium-L-deprenyl targeting astrocytes, [(18)F]fluciclatide targeting integrin α(V)β(3), [(68)Ga]IMP461 and bi-specific antibody TF2 052107 targeting carcinoembryonic antigen, [(18)F]F-metomidate targeting mitochondrial cytochrome P-450 species in the adrenal cortex, and [(18)F]vorozole targeting aromatase. Of the investigated tracers, only [(18)F]fluciclatide exhibited specific binding, whereas the other PET tracers failed to show specific uptake in the investigated tissue and are probably not useful for the intended purpose. CONCLUSION: It seems likely that α(V)β(3) integrin expression in AAA can be visualized with PET and that the α(V)β(3) selective tracer, [(18)F]fluciclatide, may be suitable for in vivo molecular imaging of asymptomatic AAA. Additional evaluation of [(18)F]fluciclatide and α(V)β(3) integrin expression in AAA will be performed in vitro as well as in vivo.
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spelling pubmed-41167622014-08-20 Autoradiography screening of potential positron emission tomography tracers for asymptomatic abdominal aortic aneurysms Tegler, Gustaf Estrada, Sergio Hall, Håkan Wanhainen, Anders Björck, Martin Sörensen, Jens Antoni, Gunnar Ups J Med Sci Original Article OBJECTIVE: The aetiology and early pathophysiological mechanisms of aortic aneurysm formation are still unknown and challenging to study in vivo. Positron emission tomography (PET) is a potentially valuable instrument for non-invasive in vivo pathophysiological studies. No specific tracer to identify the pathophysiological process of aneurysmal dilatation is yet available, however. The aim of this study was to explore if different PET tracers could be useful to image aneurysmal disease. METHODS AND RESULTS: Human aneurysmal aortic tissue, collected during elective resection of abdominal aortic aneurysm (AAA) of asymptomatic patients, was investigated in vitro by means of autoradiography with [(68)Ga]CRP-binder targeting C-reactive protein, [(11)C]DAA1106 targeting translocator protein (18 kDa), [(11)C]D-deprenyl with unknown target receptor, [(11)C]deuterium-L-deprenyl targeting astrocytes, [(18)F]fluciclatide targeting integrin α(V)β(3), [(68)Ga]IMP461 and bi-specific antibody TF2 052107 targeting carcinoembryonic antigen, [(18)F]F-metomidate targeting mitochondrial cytochrome P-450 species in the adrenal cortex, and [(18)F]vorozole targeting aromatase. Of the investigated tracers, only [(18)F]fluciclatide exhibited specific binding, whereas the other PET tracers failed to show specific uptake in the investigated tissue and are probably not useful for the intended purpose. CONCLUSION: It seems likely that α(V)β(3) integrin expression in AAA can be visualized with PET and that the α(V)β(3) selective tracer, [(18)F]fluciclatide, may be suitable for in vivo molecular imaging of asymptomatic AAA. Additional evaluation of [(18)F]fluciclatide and α(V)β(3) integrin expression in AAA will be performed in vitro as well as in vivo. Informa Healthcare 2014-08 2014-07-14 /pmc/articles/PMC4116762/ /pubmed/24555564 http://dx.doi.org/10.3109/03009734.2014.894157 Text en © Informa Healthcare http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the CC-BY-NC-ND 3.0 License which permits users to download and share the article for non-commercial purposes, so long as the article is reproduced in the whole without changes, and provided the original source is credited.
spellingShingle Original Article
Tegler, Gustaf
Estrada, Sergio
Hall, Håkan
Wanhainen, Anders
Björck, Martin
Sörensen, Jens
Antoni, Gunnar
Autoradiography screening of potential positron emission tomography tracers for asymptomatic abdominal aortic aneurysms
title Autoradiography screening of potential positron emission tomography tracers for asymptomatic abdominal aortic aneurysms
title_full Autoradiography screening of potential positron emission tomography tracers for asymptomatic abdominal aortic aneurysms
title_fullStr Autoradiography screening of potential positron emission tomography tracers for asymptomatic abdominal aortic aneurysms
title_full_unstemmed Autoradiography screening of potential positron emission tomography tracers for asymptomatic abdominal aortic aneurysms
title_short Autoradiography screening of potential positron emission tomography tracers for asymptomatic abdominal aortic aneurysms
title_sort autoradiography screening of potential positron emission tomography tracers for asymptomatic abdominal aortic aneurysms
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4116762/
https://www.ncbi.nlm.nih.gov/pubmed/24555564
http://dx.doi.org/10.3109/03009734.2014.894157
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