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MegaTevs: single-chain dual nucleases for efficient gene disruption

Targeting gene disruptions in complex genomes relies on imprecise repair by the non-homologous end-joining DNA pathway, creating mutagenic insertions or deletions (indels) at the break point. DNA end-processing enzymes are often co-expressed with genome-editing nucleases to enhance the frequency of...

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Detalles Bibliográficos
Autores principales:	Wolfs, Jason M., DaSilva, Matthew, Meister, Sarah E., Wang, Xu, Schild-Poulter, Caroline, Edgell, David R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Synthetic Biology and Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117789/ https://www.ncbi.nlm.nih.gov/pubmed/25013171 http://dx.doi.org/10.1093/nar/gku573

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