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Identification of appropriate reference genes for qPCR studies in Staphylococcus pseudintermedius and preliminary assessment of icaA gene expression in biofilm-embedded bacteria

BACKGROUND: Quantitative PCR is rapidly becoming the standard method for analyzing gene expression in a wide variety of biological samples however it can suffer from significant error if stably expressed reference genes are not identified on which to base the analysis. Suitable reference genes for q...

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Detalles Bibliográficos
Autores principales: Crawford, Evan C, Singh, Ameet, Metcalf, Devon, Gibson, Thomas WG, Weese, Scott J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118781/
https://www.ncbi.nlm.nih.gov/pubmed/25023435
http://dx.doi.org/10.1186/1756-0500-7-451