Protein crystallization with microseed matrix screening: application to human germline antibody Fabs

The crystallization of 16 human antibody Fab fragments constructed from all pairs of four different heavy chains and four different light chains was enabled by employing microseed matrix screening (MMS). In initial screening, diffraction-quality crystals were obtained for only three Fabs, while many...

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Detalles Bibliográficos
Autores principales: Obmolova, Galina, Malia, Thomas J., Teplyakov, Alexey, Sweet, Raymond W., Gilliland, Gary L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2014
Materias:
Laboratory Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118815/
https://www.ncbi.nlm.nih.gov/pubmed/25084393
http://dx.doi.org/10.1107/S2053230X14012552

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118815/
https://www.ncbi.nlm.nih.gov/pubmed/25084393
http://dx.doi.org/10.1107/S2053230X14012552

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