Long Intergenic Non-Coding RNAs (LincRNAs) Identified by RNA-Seq in Breast Cancer

In an attempt to find the correlation of aberrant expression of long intergenic noncoding RNAs (lincRNAs) with cancer, twenty-five samples of breast cancer tissue and respective adjacent normal tissue were studied for the expression of lincRNAs by RNA-seq. Among the 538 lincRNAs studied, 124 lincRNA...

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Autores principales: Ding, Xianfeng, Zhu, Limin, Ji, Ting, Zhang, Xiping, Wang, Fengmei, Gan, Shaoju, Zhao, Ming, Yang, Hongjian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118859/
https://www.ncbi.nlm.nih.gov/pubmed/25084155
http://dx.doi.org/10.1371/journal.pone.0103270
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author Ding, Xianfeng
Zhu, Limin
Ji, Ting
Zhang, Xiping
Wang, Fengmei
Gan, Shaoju
Zhao, Ming
Yang, Hongjian
author_facet Ding, Xianfeng
Zhu, Limin
Ji, Ting
Zhang, Xiping
Wang, Fengmei
Gan, Shaoju
Zhao, Ming
Yang, Hongjian
author_sort Ding, Xianfeng
collection PubMed
description In an attempt to find the correlation of aberrant expression of long intergenic noncoding RNAs (lincRNAs) with cancer, twenty-five samples of breast cancer tissue and respective adjacent normal tissue were studied for the expression of lincRNAs by RNA-seq. Among the 538 lincRNAs studied, 124 lincRNAs were exclusively expressed in cancer adjacent tissues and 62 lincRNAs were exclusively expressed in the cancer tissues. Furthermore, the expression of 134 lincRNAs was higher while 272 lower in breast cancer tissue compared with adjacent tissue. The expression of four selected lincRNAs (BC2, BC4, BC5, and BC8) was validated by semi-quantitative and real-time PCR. It was revealed that expression of lincRNA-BC5 was positively correlated with patients' age, pathological stage, and progesterone receptor concentration, while lincRNA-BC8 was negatively correlated with progesterone receptor expression. Higher expression of lincRNA-BC4 was seen in advanced breast cancer grade. LincRNA-BC2 showed no specific changes in the pathological features studied. Interactions between selected lincRNAs and breast cancer associated proteins were highly suggested by RPIseq based on the specific secondary structure. The results demonstrated that this group of lincRNAs was aberrantly expressed in breast cancer. They might play important roles in the function of oncogenes or tumor suppressors affecting the development and progression of breast cancer.
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spelling pubmed-41188592014-08-04 Long Intergenic Non-Coding RNAs (LincRNAs) Identified by RNA-Seq in Breast Cancer Ding, Xianfeng Zhu, Limin Ji, Ting Zhang, Xiping Wang, Fengmei Gan, Shaoju Zhao, Ming Yang, Hongjian PLoS One Research Article In an attempt to find the correlation of aberrant expression of long intergenic noncoding RNAs (lincRNAs) with cancer, twenty-five samples of breast cancer tissue and respective adjacent normal tissue were studied for the expression of lincRNAs by RNA-seq. Among the 538 lincRNAs studied, 124 lincRNAs were exclusively expressed in cancer adjacent tissues and 62 lincRNAs were exclusively expressed in the cancer tissues. Furthermore, the expression of 134 lincRNAs was higher while 272 lower in breast cancer tissue compared with adjacent tissue. The expression of four selected lincRNAs (BC2, BC4, BC5, and BC8) was validated by semi-quantitative and real-time PCR. It was revealed that expression of lincRNA-BC5 was positively correlated with patients' age, pathological stage, and progesterone receptor concentration, while lincRNA-BC8 was negatively correlated with progesterone receptor expression. Higher expression of lincRNA-BC4 was seen in advanced breast cancer grade. LincRNA-BC2 showed no specific changes in the pathological features studied. Interactions between selected lincRNAs and breast cancer associated proteins were highly suggested by RPIseq based on the specific secondary structure. The results demonstrated that this group of lincRNAs was aberrantly expressed in breast cancer. They might play important roles in the function of oncogenes or tumor suppressors affecting the development and progression of breast cancer. Public Library of Science 2014-08-01 /pmc/articles/PMC4118859/ /pubmed/25084155 http://dx.doi.org/10.1371/journal.pone.0103270 Text en © 2014 Ding et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ding, Xianfeng
Zhu, Limin
Ji, Ting
Zhang, Xiping
Wang, Fengmei
Gan, Shaoju
Zhao, Ming
Yang, Hongjian
Long Intergenic Non-Coding RNAs (LincRNAs) Identified by RNA-Seq in Breast Cancer
title Long Intergenic Non-Coding RNAs (LincRNAs) Identified by RNA-Seq in Breast Cancer
title_full Long Intergenic Non-Coding RNAs (LincRNAs) Identified by RNA-Seq in Breast Cancer
title_fullStr Long Intergenic Non-Coding RNAs (LincRNAs) Identified by RNA-Seq in Breast Cancer
title_full_unstemmed Long Intergenic Non-Coding RNAs (LincRNAs) Identified by RNA-Seq in Breast Cancer
title_short Long Intergenic Non-Coding RNAs (LincRNAs) Identified by RNA-Seq in Breast Cancer
title_sort long intergenic non-coding rnas (lincrnas) identified by rna-seq in breast cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118859/
https://www.ncbi.nlm.nih.gov/pubmed/25084155
http://dx.doi.org/10.1371/journal.pone.0103270
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