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The Influence of DNA Extraction Procedure and Primer Set on the Bacterial Community Analysis by Pyrosequencing of Barcoded 16S rRNA Gene Amplicons

In this study, the effect of different DNA extraction procedures and primer sets on pyrosequencing results regarding the composition of bacterial communities in the ileum of piglets was investigated. Ileal chyme from piglets fed a diet containing different amounts of zinc oxide was used to evaluate...

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Detalles Bibliográficos
Autores principales: Starke, Ingo C., Vahjen, Wilfried, Pieper, Robert, Zentek, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4120916/
https://www.ncbi.nlm.nih.gov/pubmed/25120931
http://dx.doi.org/10.1155/2014/548683