Extraction, purification, kinetic and thermodynamic properties of urease from germinating Pisum Sativum L. seeds

BACKGROUND: Urease, one of the highly efficient known enzymes, catalyzes the hydrolysis of urea into ammonia and carbon dioxide. The present study aimed to extract urease from pea seeds (Pisum Sativum L). The enzyme was then purified in three consequence steps: acetone precipitation, DEAE-cellulose...

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Detalles Bibliográficos
Autores principales: EL-Hefnawy, Mohamed E, Sakran, Mohamed, Ismail, Ali I, Aboelfetoh, Eman Fahmy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121304/
https://www.ncbi.nlm.nih.gov/pubmed/25065975
http://dx.doi.org/10.1186/1471-2091-15-15

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121304/
https://www.ncbi.nlm.nih.gov/pubmed/25065975
http://dx.doi.org/10.1186/1471-2091-15-15

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