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Methylamine-Sensitive Amperometric Biosensor Based on (His)(6)-Tagged Hansenula polymorpha Methylamine Oxidase Immobilized on the Gold Nanoparticles

A novel methylamine-selective amperometric bienzyme biosensor based on recombinant primary amine oxidase isolated from the recombinant yeast strain Saccharomyces cerevisiae and commercial horseradish peroxidase is described. Two amine oxidase preparations were used: free enzyme (AMO) and covalently...

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Detalles Bibliográficos
Autores principales: Stasyuk, Nataliya Ye., Smutok, Oleh V., Zakalskiy, Andriy E., Zakalska, Oksana M., Gonchar, Mykhailo V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124223/
https://www.ncbi.nlm.nih.gov/pubmed/25136590
http://dx.doi.org/10.1155/2014/480498