Random and targeted transgene insertion in C. elegans using a modified Mosl transposon

We have generated a recombinant Mos1 transposon that can insert up to 45 kb transgenes into the C. elegans genome. The minimal Mos1 transposon (miniMos) is 550 bp long and inserts DNA into the genome at high frequency (~60% of injected animals). Genetic and antibiotic markers can be used for selecti...

Descripción completa

Detalles Bibliográficos
Autores principales: Frokjaer-Jensen, Christian, Davis, M Wayne, Sarov, Mihail, Taylor, Jon, Flibotte, Stephane, LaBella, Matthew, Pozniakovski, Andrei, Moerman, Donald G, Jorgensen, Erik M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4126194/
https://www.ncbi.nlm.nih.gov/pubmed/24820376
http://dx.doi.org/10.1038/nmeth.2889

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4126194/
https://www.ncbi.nlm.nih.gov/pubmed/24820376
http://dx.doi.org/10.1038/nmeth.2889

Ejemplares similares