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A three-dimensional collagen construct to model lipopolysaccharide-induced activation of BV2 microglia
BACKGROUND: We report a novel method of culturing microglia in three dimension (3D) using collagen as a substrate. By culturing microglia within a matrix, we aim to emulate the physical state of microglia embedded within parenchyma. METHODS: BV2 microglia cell suspensions were prepared with type I c...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4128540/ https://www.ncbi.nlm.nih.gov/pubmed/25074682 http://dx.doi.org/10.1186/1742-2094-11-134 |