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Protein interaction mapping with ribosome-displayed using PLATO ORF libraries

Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by mass...

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Detalles Bibliográficos
Autores principales: Zhu, Jian, Larman, H. Benjamin, Gao, Geng, Somwar, Romel, Zhang, Zijuan, Laserson, Uri, Ciccia, Alberto, Pavlova, Natalya, Church, George, Zhang, Wei, Kesari, Santosh, Elledge, Stephen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129458/
https://www.ncbi.nlm.nih.gov/pubmed/24336473
http://dx.doi.org/10.1038/nprot.2013.167