Protein interaction mapping with ribosome-displayed using PLATO ORF libraries

Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by mass...

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Detalles Bibliográficos
Autores principales: Zhu, Jian, Larman, H. Benjamin, Gao, Geng, Somwar, Romel, Zhang, Zijuan, Laserson, Uri, Ciccia, Alberto, Pavlova, Natalya, Church, George, Zhang, Wei, Kesari, Santosh, Elledge, Stephen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129458/
https://www.ncbi.nlm.nih.gov/pubmed/24336473
http://dx.doi.org/10.1038/nprot.2013.167
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author Zhu, Jian
Larman, H. Benjamin
Gao, Geng
Somwar, Romel
Zhang, Zijuan
Laserson, Uri
Ciccia, Alberto
Pavlova, Natalya
Church, George
Zhang, Wei
Kesari, Santosh
Elledge, Stephen J.
author_facet Zhu, Jian
Larman, H. Benjamin
Gao, Geng
Somwar, Romel
Zhang, Zijuan
Laserson, Uri
Ciccia, Alberto
Pavlova, Natalya
Church, George
Zhang, Wei
Kesari, Santosh
Elledge, Stephen J.
author_sort Zhu, Jian
collection PubMed
description Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by massively parallel analysis using DNA sequencing. We demonstrate the broad utility of the method by identifying known and new interacting partners of LYN kinase, patient autoantibodies and the small molecules gefitinib and dasatinib.
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spelling pubmed-41294582014-08-12 Protein interaction mapping with ribosome-displayed using PLATO ORF libraries Zhu, Jian Larman, H. Benjamin Gao, Geng Somwar, Romel Zhang, Zijuan Laserson, Uri Ciccia, Alberto Pavlova, Natalya Church, George Zhang, Wei Kesari, Santosh Elledge, Stephen J. Nat Protoc Article Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by massively parallel analysis using DNA sequencing. We demonstrate the broad utility of the method by identifying known and new interacting partners of LYN kinase, patient autoantibodies and the small molecules gefitinib and dasatinib. 2013-12-12 2014-01 /pmc/articles/PMC4129458/ /pubmed/24336473 http://dx.doi.org/10.1038/nprot.2013.167 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Zhu, Jian
Larman, H. Benjamin
Gao, Geng
Somwar, Romel
Zhang, Zijuan
Laserson, Uri
Ciccia, Alberto
Pavlova, Natalya
Church, George
Zhang, Wei
Kesari, Santosh
Elledge, Stephen J.
Protein interaction mapping with ribosome-displayed using PLATO ORF libraries
title Protein interaction mapping with ribosome-displayed using PLATO ORF libraries
title_full Protein interaction mapping with ribosome-displayed using PLATO ORF libraries
title_fullStr Protein interaction mapping with ribosome-displayed using PLATO ORF libraries
title_full_unstemmed Protein interaction mapping with ribosome-displayed using PLATO ORF libraries
title_short Protein interaction mapping with ribosome-displayed using PLATO ORF libraries
title_sort protein interaction mapping with ribosome-displayed using plato orf libraries
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129458/
https://www.ncbi.nlm.nih.gov/pubmed/24336473
http://dx.doi.org/10.1038/nprot.2013.167
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