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Protein interaction mapping with ribosome-displayed using PLATO ORF libraries
Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by mass...
Autores principales: | Zhu, Jian, Larman, H. Benjamin, Gao, Geng, Somwar, Romel, Zhang, Zijuan, Laserson, Uri, Ciccia, Alberto, Pavlova, Natalya, Church, George, Zhang, Wei, Kesari, Santosh, Elledge, Stephen J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129458/ https://www.ncbi.nlm.nih.gov/pubmed/24336473 http://dx.doi.org/10.1038/nprot.2013.167 |
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